SW 1417

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90102543, human colon, Epithelial

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human colon
• envase: tube of 5 μg 90102543-DNA-5UG; pkg of vial of cells 90102543-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: Epithelial
• productos: Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human colon adenocarcinoma

Descripción línea celular

Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.

Aplicación

Tumourigenicity studies

Medio de cultivo

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Centrifugation and resuspension are recommended to disperse clumps.

Otras notas

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