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C0412

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Sinónimos:

Con A

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5 MG
$68.54

About This Item

Número de CAS:
11028-71-0
Número CE:
234-258-2
Número MDL:
MFCD00071069
Código UNSPSC:
23151817
NACRES:
NA.32

$68.54

Precio de catálogo$73.30Ahorre 6 %
Web-Only Promotion

Disponible para envío el07 de abril de 2025Detalles

Nivel de calidad

200

esterilidad

γ-irradiated

tpo

Type IV-S

Línea del producto

BioReagent

Formulario

lyophilized powder

técnicas

cell culture | mammalian: suitable

impurezas

salt, essentially free

solubilidad

PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy

temp. de almacenamiento

−20°C

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Categorías relacionadas

Aplicación

Concanavalin A from Canavalia ensiformis (jack bean) has been used:
  • to induce spleen blastogenesis[1]
  • to generate ConA blast targets from B6 and lpr spleen cells[2]
  • to stimulate splenic lymphocytes from estrogen-and placebo-treated mice[3]

Acciones bioquímicas o fisiológicas

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Nota de análisis

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H317,H334,H361

Clasificaciones de peligro

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SLCP7735
SLCB0143
SLBT4976
SLBN5209V
SLBJ2854V

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Questions

  1. ConA 제품 (C0412)에 관해 질문 드립니다. ConA가 Ca2+, Mn2+ 이온이 존재해야 활성화가 된다고 하는데 해당 제품은 powder형태이며 이는 따로 용액이 필요합니다. 그렇다면 이 제품 안에 저 금속 이온이 함께 들어있는 건지, 아니면 Sigma 제품 중에 이 제품을 혼합할 수 있는 PBS가 따로 있는지(있다면 카탈로그 번호 알려주셨으면 합니다) 궁금합니다.

    1 answer

    1. Concanavalin A has 2 metal binding sites per monomer. Calcium and Manganese are required to bind these sites in order for sugar binding to occur. It is recommended to prepare stock solutions in PBS containing Ca2+ and Mn2+.

      See the link below to the product datasheet which includes preparation instructions:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/331/341/c0412dat.pdf

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