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MilliporeSigma

L8902

Sigma-Aldrich

Lectina de Phaseolus vulgaris (frijol rojo)

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Sinónimos:

Aglutinina de Phaseolus vulgaris, PHA

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About This Item

EC Number:
UNSPSC Code:
12352202
NACRES:
NA.32

Quality Level

product line

BioReagent

form

lyophilized powder

potency

<40 μg per mL agglutination activity

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

PBS: 2 mg/mL

storage temp.

2-8°C

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Application

Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.

Biochem/physiol Actions

La PHA consta de dos especies moleculares, la eritroaglutinina (PHA-E), que tiene poca actividad mitógena y elevada actividad de eritroaglutinina, y la leoucoaglutinina (PHA-L), que tiene elevada actividad mitógena y leoucoaglutinante, pero muy baja actividad eritroaglutininante
La PHA-E no es específica de grupo sanguíneo, pero la aglutinación puede ser inhibida por ciertos oligosacáridos. La PHA-P es la forma proteica de la PHA antes de la separación y la purificación de la eritroaglutinina y la leoucoaglutinina. La PHA-M es la forma mucoproteínica. Los conjugados se preparan a partir de las correspondientes lectinas purificadas.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

related product

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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