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A2404

Sigma-Aldrich

Alcohol Oxidase solution from Pichia pastoris

buffered aqueous solution, 10-40 units/mg protein (biuret)

Sinónimos:

Alcohol:oxygen oxidoreductase

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About This Item

Número de CAS:
9073-63-6
Comisión internacional de enzimas:
1.1.3.13 (BRENDA, IUBMB)
Número MDL:
MFCD00130452
Código UNSPSC:
12352204
NACRES:
NA.54

Formulario

buffered aqueous solution

actividad específica

10-40 units/mg protein (biuret)

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.

Aplicación

Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones .
Alcohol Oxidase solution from Pichia pastoris has been used in cotton pectin methylesterase (PME) enzyme assay. It has also been used to immobilize onto polyaniline (PANI) film as a visual biosensor in dip-stick format for the determination of ethanol in fermented beverage samples.

Acciones bioquímicas o fisiológicas

Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.

Definición de unidad

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

Forma física

Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000390827
0000343854
0000343855
0000299797
0000299797

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Henry Temple et al.
Nature plants, 8(6), 656-669 (2022-06-11)
Polysaccharide methylation, especially that of pectin, is a common and important feature of land plant cell walls. Polysaccharide methylation takes place in the Golgi apparatus and therefore relies on the import of S-adenosyl methionine (SAM) from the cytosol into the
Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties
Liu Q, et al.
PLoS ONE, 8(6), e65131-e65131 (2013)
Xuan Wang et al.
Journal of experimental botany, 71(9), 2629-2640 (2020-02-02)
De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by
Turn air-captured CO2 with methanol into amino acid and pyruvate in an ATP/NAD(P)H-free chemoenzymatic system.
Liu, et al.
Nature Communications, 14, 2772-2772 (2023)
A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples
Kuswandi B, et al.
Sensors, 14(2), 2135-2149 (2014)
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Protocolos

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.

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