The activity listed on the Certificate of Analysis for each lot can be used to convert from units to milligrams of solid.
P8375
Peroxidasa from horseradish
Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)
Sinónimos:
Donante:peróxido de hidrógeno oxidorreductasa, Peroxidasa de rábano picante
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1000 units | Disponible para enviar HOYdesdeMILWAUKEE | $72.70 |
2000 units | Disponible para enviar HOYdesdeMILWAUKEE | $118.00 |
5000 units | Disponible para enviar HOYdesdeMILWAUKEE | $135.00 |
10000 units | Disponible para enviar HOYdesdeMILWAUKEE | $291.00 |
25000 units | Disponible para enviar HOYdesdeMILWAUKEE | $497.00 |
100000 units | Disponible para enviar HOYdesdeMILWAUKEE | $2,100.00 |
250000 units | Fecha estimada de envío26 de octubre de 2026desdeMILWAUKEE | $4,030.00 |
Acerca de este artículo
Número CAS:
eCl@ss:
32160410
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-668-6
MDL number:
Número CE:
Specific activity:
≥250 units/mg solid (using pyrogallol)
Servicio técnico
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Type VI
Quality Segment
form
essentially salt-free, lyophilized powder
specific activity
≥250 units/mg solid (using pyrogallol)
mol wt
~44 kDa
technique(s)
protein detection: suitable
solubility
0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)
absorbance ratio
RZ 2.5-4.0
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
General description
La peroxidasa de rábano picante se aísla de las raíces de rábano picante (Amoracia rusticana) y pertenece al grupo de ferroprotoporfirina de las peroxidasas. La HRP es un polipéptido monocatenario que contiene cuatro puentes bisulfuro. Es una glucoproteína que contiene un 18 % de carbohidratos. La composición de los carbohidratos consiste en galactosa, arabinosa, xilosa, fucosa, manosa, manosamina y galactosamina dependiendo de la isoenzima específica. Su peso molecular (~44 kDa) incluye la cadena polipeptídica (33 890 dalton), la hemina más Ca2+ (~700 dalton) y los carbohidratos (~9 400 dalton). Existen al menos siete isoenzimas de la HRP. El punto isoeléctrico de las isoenzimas de la peroxidasa de rábano picante oscila entre 3,0 y 9,0.
Application
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases.. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8375, type VI, is an essentially salt free lyophilized powder. It is commonly used to determine amounts of glucose and peroxides in solution. It has been used to study sensory input of cervical spinal cord neurons.
Biochem/physiol Actions
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant. Known inhibitors are sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase and hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.
Analysis Note
La RZ (Reinheitszahl) es la relación de absorbancia A403/A275 determinada a 0,5-1,0 mg/ml en agua desionizada. Es una medida del contenido de hemina, no de la actividad enzimática. Incluso preparaciones con RZ elevada pueden tener una actividad enzimática baja.
Other Notes
Una unidad de pirogalol formará 1,0 mg de purpurogalina a partir de pirogalol en 20 segundos a pH 6,0 a 20 °C.
Véase más información sobre la peroxidasa en www.sigma-aldrich.com/enzymeexplorer.
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Este artículo | |||
|---|---|---|---|
| description Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol) | description lyophilized, powder, ~150 U/mg | description Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol) | description Type XII, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol) |
| technique(s) protein detection: suitable | technique(s) protein detection: suitable | technique(s) protein detection: suitable | technique(s) protein detection: suitable |
| specific activity ≥250 units/mg solid (using pyrogallol) | specific activity ~150 U/mg | specific activity 150-250 units/mg solid (using pyrogallol) | specific activity ≥250 units/mg solid (using pyrogallol) |
| form essentially salt-free, lyophilized powder | form powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility 0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0) | solubility 50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA) | solubility H2O: soluble, 0.1 M phosphate buffer: soluble (pH 6.0) | solubility H2O: soluble, 0.1 M phosphate buffer: soluble (pH 6.0) |
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Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 1
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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How do I convert units of Peroxidase from horseradish, Product P8375, to solid?
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What is the optimal pH range of Peroxidase from horseradish, Product P8375?
1 answer-
The optimal pH range for product P8375 is 6.0 - 6.5.
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What can you tell me about the stability of Peroxidase from horseradish, Product P8375, (as is, in suspension and in solution)?
1 answer-
This product is stable as a powder, kept refrigerated. See the certificate of analysis for a lot-specific recommended retest date. It is also stable as a crystalline suspension of 3.2 M (NH4)2SO4 solution containing potassium phosphate buffer, pH 6.0 (see Product No. P6140). It is reasonably stable in solution in phosphate buffer. We have found that at 10 mg/mL in 0.1 phosphate buffer, pH 6.0, solutions kept frozen at -20°C lose less than 2% of their activity per week.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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