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86R

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast red violet LB

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1 KIT
$215.00

$215.00

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1 KIT
$215.00

About This Item

Código UNSPSC:
12352106
eCl@ss:
42010105
NACRES:
NA.47

$215.00

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Nivel de calidad

500

caducidad

Expiry date on the label.

DIV

for in vitro diagnostic use

dilution

(for histology)

aplicaciones

hematology
histology

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

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Aplicación

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast red violet LB. The resulting insoluble diffuse, red dye deposit indicates sites of alkaline phosphatase activity.

Solo componentes del kit

Referencia del producto
Descripción
  • Citrate Solution (915) 50 mL
  • FRV-Alkaline Solution (862) 10 mL
  • Hematoxylin Solution, Gill No. 3 (kit only) 50 mL
  • Naphthol AS-BI Alkaline Solution (861) 10 mL
  • Sodium Nitrite Solution (914) 10 mL

Palabra de señalización

Danger

Frases de peligro

H290,H302,H315,H318,H373

Clasificaciones de peligro

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - STOT RE 2 Oral

Órganos de actuación

Kidney

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000322885
0000283230
0000283230
0000322885
0000251519

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Clive H Glover et al.
PLoS computational biology, 2(11), e158-e158 (2006-11-24)
Stem cell differentiation involves critical changes in gene expression. Identification of these should provide endpoints useful for optimizing stem cell propagation as well as potential clues about mechanisms governing stem cell maintenance. Here we describe the results of a new
Terence P Gade et al.
PloS one, 6(7), e22608-e22608 (2011-07-30)
The purpose of this study was to develop a paradigm for quantitative molecular imaging of bone cell activity. We hypothesized the feasibility of non-invasive imaging of the osteoblast enzyme alkaline phosphatase (ALP) using a small imaging molecule in combination with
Alessandro Polini et al.
PloS one, 6(10), e26211-e26211 (2011-10-25)
The development of a new family of implantable bioinspired materials is a focal point of bone tissue engineering. Implant surfaces that better mimic the natural bone extracellular matrix, a naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages
Raha Favaedi et al.
The International journal of developmental biology, 60(4-6), 103-110 (2016-07-09)
Histone H3 lysine 9 methylation has been shown to be a critical barrier to efficient cell reprogramming. This discovery allows the assessment of the cell pluripotency state by considering the extent of H3K9 methylation vs. acetylation at the same position.
Edgardo Rodríguez-Carballo et al.
PloS one, 9(7), e102032-e102032 (2014-07-10)
p38 MAPK activity plays an important role in several steps of the osteoblast lineage progression through activation of osteoblast-specific transcription factors and it is also essential for the acquisition of the osteoblast phenotype in early development. Although reports indicate p38
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Questions

1–2 of 2 Questions  

  1. iPS細胞から分化誘導された骨の凍結切片をこのキットで染色すると骨は染まりますか？

    1 answer

    1. The 86C and 86R kits have not been validated for use with frozen sections. The Specimen Collection section of the Instructions For Use advises the kit is intended for "Fresh blood or bone marrow films or samples anticoagulated with heparin may be used. AVOID EDTA." Customers working with cultured cells report the kit does seem to work. This finding has not been confirmed by our company. If the frozen sections have been decalcified, the expectation is that the kit will not work since it is likely the alkaline phosphatase enzyme was destroyed during the decalcification process, There are microtomes that can cut undecalcified bone, although they are not in common use. Really not sure if there are cryostats available that have the ability to cut undecalcified bone. If such a cryostat exists, in theory, the kit might work if the frozen sections are stained immediately after the cutting of the frozen sections. The alkaline phosphatase enzyme is not preserved at -20°C. Were the frozen sections to be stored at -80°C, there is the possibility the alkaline phosphatase enzyme might be preserved. Again there has been no validation for whether the kit can be used to stain frozen sections.

      Helpful?

  2. Can the Alkaline Phosphatase kits in 85 or 85 series kits be used to stain paraffin embedded sections of bone?

    1 answer

    1. The 86R kit, along with its possible replacements (85L1, 85L2, and 86C), is currently not available. These kits, part of the 85 and 86 series, are typically intended for use with peripheral blood and bone marrow. The Instructions for Use advise using fresh blood or bone marrow films anticoagulated with heparin, avoiding EDTA. Blood smears should be stained for enzyme activity within 8 hours after preparation, and films should be dried at least 1 hour before fixation and 3 hours post-fixation before freezing. While these kits are designated for peripheral blood and bone marrow, some users have utilized them for cultured cells and deparaffinized bone. However, the sensitivity of these kits for detecting alkaline phosphatase activity in paraffin sections of bone may be limited.

      Helpful?

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