Saltar al contenido
MilliporeSigma
Todas las fotos(1)

Documentos clave

17-128

Sigma-Aldrich

Alkaline/Acid Phosphatase Assay Kit (R-R-A-pS-V-A)

Alkaline/Acid Phosphatase Assay Kit is routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP.

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización

Seleccione un Tamaño

1 KIT
$620.00

$620.00

Fecha estimada de envío28 de mayo de 2025

Todas las fotos(1)

Seleccione un Tamaño

Cambiar Vistas
1 KIT
$620.00

About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

$620.00

Fecha estimada de envío28 de mayo de 2025

Nivel de calidad

100

fabricante / nombre comercial

Upstate®

técnicas

activity assay: suitable (phosphatase)

método de detección

colorimetric

Condiciones de envío

wet ice

Aplicación

Used to detect/quantify: Alkaline/Acid

Envase

Kit capacity: 100 assays

Componentes

Malachite Green Solution A (Cat.# 20-105)

Malachite Green Additive (Cat.# 20-104)

Phosphate Standard (Cat.# 20-103)

Serine Phosphopeptide (RRApSVA) (Cat.# 12-220)

pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)

NiCl2, 40mM (Cat.# 20-178)

pNPP Ser/Thr Assay Buffer (Cat.# 20-179)

96-well microtiter plate

Calidad

routinely used to detect phosphatase activity by either dephosphorylation of the phosphopeptide (RRApSVA) or hydrolysis of pNPP

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Palabra de señalización

Danger

Clasificaciones de peligro

Aquatic Chronic 3 - Carc. 1A Inhalation - Met. Corr. 1 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Órganos de actuación

Lungs

Código de clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 3

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

An investigation of the substrate specificity of protein phosphatase 2C using synthetic peptide substrates; comparison with protein phosphatase 2A
Donella Deana, A., et al
Biochimica et Biophysica Acta, 1051, 199-202 (1990)
Nathan J Castro et al.
Tissue engineering. Part A, 22(13-14), 940-948 (2016-06-15)
Osseous tissue defects caused by trauma present a common clinical problem. Although traditional clinical procedures have been successfully employed, several limitations persist with regards to insufficient donor tissue, disease transmission, and inadequate host-implant integration. Therefore, this work aims to address
Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green.
Ekman, P and Jager, O
Analytical biochemistry, 214, 138-141 (1993)
P P Van Veldhoven et al.
Analytical biochemistry, 161(1), 45-48 (1987-02-15)
A procedure, based on the complex formation of malachite green with phosphomolybdate under acidic conditions, to measure inorganic orthophosphate in the nanomolar range is described. The addition of polyvinyl alcohol is required to stabilize the dye-phosphomolybdate complex. The advantages of
A Donella-Deana et al.
Biochimica et biophysica acta, 1094(1), 130-133 (1991-08-13)
The four main classes of protein phosphatases (PP-1, 2A, 2B and 2C), although differing in their ability to dephosphorylate phosphopeptide substrates, invariably display a marked preference toward phosphothreonyl peptides over their phosphoseryl counterparts. Conversely, all the acidic and alkaline phosphatases
Ver todos los artículos relacionados

Questions

Reviews

No rating value

Active Filters

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico