ROAMYGLL

Amyloglucosidase

from Aspergillus niger

• Sinónimos: Aspergillus niger, disaccharidase-type a-glucosidase
• Comisión internacional de enzimas: 3.2.1.3 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: Aspergillus niger
• Nivel de calidad: 100
• Formulario: lyophilized
• actividad específica: 6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate); ~6 units/mg protein (At 25 °C with glycogen as the substrate.)
• mol peso: 97 kDa
• envase: pkg of 3,500 U (11202367001); pkg of 500 U (11202332001)
• fabricante / nombre comercial: Roche
• pH óptimo: 4.6-4.8
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase.[1] It is synthesized by several Aspergillus genus species.[2] This exo-enzyme is one of the major industrial enzymes.[3] The stability of amyloglucosidase can be increased by immobilization.[3]

Especificidad

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Aplicación

Amyloglucosidase from Aspergillus niger is used for the determination of starch.[4]

Acciones bioquímicas o fisiológicas

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose.[5][6] The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.[7]

Nota de preparación

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:

• Western blot: 1 to 10 μg/ml

Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash