MABE163

Anti-ASF-1 (SF2) Antibody, clone AK96

clone AK96, from mouse

• Sinónimos: splicing factor, arginine/serine-rich 1, Alternative-splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, splicing factor 22, ASF-1, SF2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: AK96, monoclonal
• reactividad de especies: human
• reactividad de especies (predicha por homología): rat (immunogen homology), mouse (immunogen homology)
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_001071634
• Nº de acceso UniProt: Q07955
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SRSF1(6426)

Descripción

Descripción general

Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

Especificidad

This antibody recognizes the N-terminus of ASF-1 (SF2).

Inmunógeno

Epitope: N-terminus

MBP-tagged recombinant protein corresponding to the N-terminus of human ASF-1 (SF2).

Aplicación

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ASF-1 (SF2) in human prostate carcinoma cells and human testis tissue.
Western Blotting Analysis: A representative lot was used by an independent laboratory in western blotting (Sinha, R., et al. (2010). Mol. Cell. Biol. doi:10.1128/MCB.01270-09).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 (SF2) in HeLa nuclear extracts (Jablonski, J. A., et al. (2010). Nucleic Acids Research. 38(4):1249-1260).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-ASF-1 (SF2) Antibody, clone AK96 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC to detect ASF-1 (SF2) also known as splicing factor arginine/serine-rich 1, Alternative-splicing factor 1 & pre-mRNA-splicing factor SF2 P33 subunit

Calidad

Evaluated by Western Blot in human lymph node lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ASF-1 (SF2) on 10 µg of human lymph node lysate.

Descripción de destino

~ 30 kDa observed

Ligadura / enlace

Replaces: MABE512

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Human lymph node lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable