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GR32L

Sigma-Aldrich

Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)

lyophilized, clone BuGR2, Calbiochem®

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100 μG
$609.00

$609.00


Fecha estimada de envío28 de mayo de 2025


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100 μG
$609.00

About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

$609.00


Fecha estimada de envío28 de mayo de 2025


Solicitar un pedido a granel

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

BuGR2, monoclonal

Formulario

lyophilized

contiene

≤0.1% sodium azide as preservative

reactividad de especies

guinea pig, rabbit, rat, mouse, sheep

no debe reaccionar con

amphibian, primate, avian

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

isotipo

IgG2a

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

mouse ... Nr3C1(14815)

Descripción general

Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.
Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.

Inmunógeno

Rat
partially purified, rat glucocorticoid receptor

Aplicación


Frozen Sections (5 g/ml)
Immunoblotting (5 g/ml)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 g/ml)

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from PBS, pH 7.2.

Reconstitución

Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Nota de análisis

Positive Control
mouse liver extract

Otras notas

Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 1521.
Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.
Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.
Weigel, N.L. 1996. Biochem J.319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mohammad M Khan et al.
Neuroendocrinology, 84(5), 317-329 (2006-12-05)
MNAR/PELP1 (see text) is a newly identified scaffold protein/coactivator initially thought to modulate nongenomic and genomic actions of the estrogen receptor; however, it has been recently shown to interact with multiple steroid receptors, including androgen and glucocorticoid receptors. In the
Biswajyoti Sahu et al.
Cancer research, 73(5), 1570-1580 (2012-12-28)
The forkhead protein FoxA1 has functions other than a pioneer factor, in that its depletion brings about a significant redistribution in the androgen receptor (AR) and glucocorticoid receptor (GR) cistromes. In this study, we found a novel function for FoxA1
Nina M Patrick et al.
The Journal of steroid biochemistry and molecular biology, 167, 1-13 (2016-09-21)
Small molecule inhibitors of lysine deacetylases (KDACs) are approved for clinical use in treatment of several diseases. Nuclear receptors, such as the glucocorticoid receptor (GR) use lysine acetyltransferases (KATs or HATs) and KDACs to regulate transcription through acetylation and deacetylation
Vineela Kadiyala et al.
The Journal of biological chemistry, 288(40), 28900-28912 (2013-08-16)
Nuclear receptors use lysine acetyltransferases and lysine deacetylases (KDACs) in regulating transcription through histone acetylation. Lysine acetyltransferases interact with steroid receptors upon binding of an agonist and are recruited to target genes. KDACs have been shown to interact with steroid

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