FCMAB193F

Milli-Mark^® Anti-CD33-FITC Antibody, clone WM-54

clone WM-54, Milli-Mark^®, from mouse

• Sinónimos: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: FITC conjugate
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: WM-54, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Milli-Mark^®
• técnicas: flow cytometry: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001763.3
• Nº de acceso UniProt: P20138
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CD33(945)

Descripción

Descripción general

CD33 is a member of the siglec family (sialic acid binding immunoglobulin-like lectins) referred to as Siglec-3 (Freeman SD, Kelm S, Barber EK and Crocker PR, 1995, Blood, 2005-2012). It is the smallest member of the siglec family having a molecular weight of 67 kDa (Peiper SC, Ashmun RA and Look TA (1988) Blood., 72: 314-321). CD33 mediates protein-carbohydrate cellular interactions in addition to protein-protein recognition (Crocker, 2002). The CD33 gene is located on chromosome 19 (Peiper, 1997). CD33 is expressed by subsets of monocytes and neutrophils. CD33 is the major antigen to differentially diagnose myeloid from lymphoid leukemias, as it is expressed on 90% of myeloid and not lymphoid leukemic cells (Andrews 1989; Griffin 1984; and Sabbath 1986). Committed hemapoietic progenitors, including multipotential stem cells (CFU-GEMM) and precursors of granulocytes and macrophages (CFU-GM) and early erythroid progenitors (BFU-E) express CD33 (Peiper, 1989 and 1997). Anti-CD33, WM-54, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD33 (Peiper, 1989).

Especificidad

Antibody recognizes Human CD33 antigen.

Inmunógeno

Leukemic cells from a patient with AML.

Aplicación

Milli-Mark Anti-CD33-FITC Antibody, clone WM-54 detects level of CD33 & has been published & validated for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Calidad

Evaluated by flow cytometry using human PBMCs

Descripción de destino

67 kDa (Peiper, 1997)

Forma física

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Almacenamiento y estabilidad

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Nota de análisis

Control
PBMCs

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable