539305
Protein G, Alkaline Phosphatase Conjugate
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500 μG
$250.00
$250.00
Fecha estimada de envío26 de marzo de 2025
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500 μG
$250.00
About This Item
UNSPSC Code:
41116133
NACRES:
NA.41
$250.00
Fecha estimada de envío26 de marzo de 2025
Productos recomendados
form
liquid
Quality Level
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
shipped in
wet ice
storage temp.
2-8°C
General description
Protein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.
Protein G, Alkaline Phosphatase Conjugate is obtained by conjugation of protein G, a cell-surface protein of Streptococcus species to alkaline phosphatase. This conjugate is used as a secondary agent[1] in immunoassay methods to detect polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a, and IgG2b.[2]
Application
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
Analysis Note
Single band at 33.8 kDa by SDS-PAGE
Other Notes
p-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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B Nilson et al.
Journal of immunoassay, 9(2), 207-225 (1988-01-01)
Protein G, an IgG-binding protein, purified from the surface of group G streptococci, was coupled to alkaline phosphatase. The conjugate was used for detection of polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a and IgG2b in an enzyme-linked
S Sun et al.
Journal of immunological methods, 152(1), 43-48 (1992-07-31)
The IgG binding domains of staphylococcal protein A and streptococcal protein G were expressed as a chimaera using the pGEX vector which has been advocated because its fusion proteins tend to be soluble and easily isolated on immobilised glutathione. This
A O Olarinmoye et al.
Epidemiology and infection, 145(10), 2030-2037 (2017-05-10)
The hamadryas baboon (Papio hamadryas hamadryas) is the only indigenous species of non-human primates (NHP) found in the Kingdom of Saudi Arabia (KSA). There are no peer-reviewed publications on viral infections of the baboons of KSA. Apart from camels, other
Anja Reckendorf et al.
International journal for parasitology. Parasites and wildlife, 14, 53-61 (2021-01-26)
Pseudaliid lungworm (Metastrongyloidea) infections and associated secondary bacterial infections may severely affect the health status of harbour porpoises (Phocoena phocoena) in German waters. The presented retrospective analysis including data from 259 harbour porpoises stranded between 2006 and 2018 on the
B N Ogunro et al.
Journal of epidemiology and global health, 8(3-4), 162-170 (2019-03-14)
Ebola viruses (family: Filoviridae) are the cause of Ebola virus disease (EVD), a highly fatal illness characterised by haemorrhagic fever syndrome in both humans and non-human primates (NHPs). West Africa was the epicentre of the 2013-2015 EVD epidemic which caused
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