Histopaque 10771 was introduced in the late 1970s or early 1980s. As such, there is a long history of shipping the product when the temperature falls below freezing. It is relatively common for the bottles to freeze during ground shipping during the winter months. The standard advise is to thaw the bottles at either room temperature at a 37°C. Once the bottle has thawed, it will be necessary to invert the bottle multiple times to ensure the gradient is uniform. If the bottle is not inverted multiple times, a gradient in situ will form as freezing is one method of forming gradients. As long as the solution is clear and the bottle has not broken, the product is suitable for use.
10771
Histopaque®-1077
sterile-filtered, density: 1.077
Histopaque®-1077
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
100 mL | Comprobar disponibilidad del carrito | $61.20 |
500 mL | Comprobar disponibilidad del carrito | $227.00 |
6 x 100 mL | Comprobar disponibilidad del carrito | $271.00 |
Acerca de este artículo
NACRES:
NA.75
UNSPSC Code:
12352207
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlesterility
sterile-filtered
Quality Segment
form
liquid
shelf life
Expiry date on the label.
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
density
1.077
application(s)
hematology
histology
storage temp.
2-8°C
General description
Histopaque-1077 es un medio estéril, probado para endotoxinas y listo para usar. La composición de Histopaque consiste en polisacarosa y diatrizoato de sodio, ajustados a una densidad de 1,077 g/ml. Este medio listo para usar facilita la recuperación rápida de los linfocitos viables y otras células mononucleares de pequeños volúmenes de sangre completa o médula ósea.
Application
Histopaque-1077 se ha utilizado para:
- la determinación de los antígenos del complejo principal de histocompatibilidad (HLA)
- como etapa inicial del aislamiento antes del recuento de linfocitos T, B y ‘nulos’.
- preparación de suspensiones puras de linfocitos para cultivos celulares y ensayos de citotoxicidad.
- Aislamiento y purificación de células mononucleares de sangre periférica (PBMC) y células mononucleares de médula ósea (BMMC)[1]
- aislamiento de linfocitos humanos
- aislamiento de células tumorales circulantes de cáncer de mama (CTC) en sangre completa[2]
- Histopaque-1077 se utiliza junto con Histopaque-1119 ya que facilita la recuperación rápida de células mononucleares viables y granulocitos de pequeños volúmenes de sangre completa
Biochem/physiol Actions
La sangre anticoagulada se coloca en capas sobre el Histopaque-1077. Durante la centrifugación, la polisacarosa produce agregación de los eritrocitos, que sedimentan rápidamente. Los granulocitos se vuelven ligeramente hipertónicos, lo que aumenta su velocidad de sedimentación y provoca su depósito en el fondo del tubo de centrífuga. Los linfocitos y otras células mononucleares permanecen en la interfaz plasma/Histopaque-1077.
Features and Benefits
Histopaque®-1077 ofrece numerosas ventajas, como:
- Recuperación óptima de las células viables
- Permite la separación selectiva de los tipos de células sanguíneas
- Previene la distorsión celular
- Preserva la viabilidad celular
- Interferencia mínima de células extrañas
- Rendimiento reproducible de lote a lote
- Esterilizado mediante filtración y probado para endotoxinas
- Estable a 2-8°C durante al menos 2 años cuando está protegido de la luz
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Este artículo | |||
|---|---|---|---|
| density 1.077 | density 1.083 | density 1.077 at 25 °C (relative density)(lit.) | density 1.077 g/mL |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form solution |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life - | shelf life Expiry date on the label. |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
12 - Non Combustible Liquids
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
-
Hello, we had a problem with the refrigerator temperature dropping below 4 degrees Celsius for over the weekend. Can the Histopaque-1077 stored in this refrigerator still be used?
1 answer-
Helpful?
-
-
Hello, we had a problem with the refrigerator leading the temperature increasing to 44 degrees Celsius for over the 2 days of weekend, so can our Histopaque-1077 stored in this refrigerator can be used anymore?
1 answer-
This product ships at ambient temperature and is stable for relatively short periods at room temperature. There is a 1 - 2 week allowance for transit time. A storage temperature excursion of 2 days is not expected to impact the quality or performance of this material.
Helpful?
-
-
Is Histopaque 10771 the recommended density for isolating or separating PBMCs in llama blood?
1 answer-
Yes, Histopaque 1077 (catalog number 10771) is the recommended density for mammalian cells.
Helpful?
-
-
Can Histopaque 10771 be placed in a 37°C water bath to bring it to room temperature after being taken out of the refrigerator?
1 answer-
Histopaque 10771 is normally stored at 2-8°C for long-term storage. However, it can be shipped at ambient temperature, suggesting that it is not easily affected by short-term storage conditions. Therefore, warming it up to 37°C is unlikely to have any detrimental effects on the product.
Helpful?
-
-
Can we use a ratio of blood to separation media of 4:3 with Histopaque-1077? Also, can Histopaque-1077 be used to dilute the blood up to 1:2 before layering with separation media with HBSS buffer, and then wash the cells too.
1 answer-
Histopaque 10771/H8889 and Ficoll Paque solutions are produced with similar reagents. As Histopaque has a slightly lower density range, yields might be slightly lower. Therefore, it is a mixed bag and yields with Histopaque might be slightly lower. When observing a very high white cell count, dilution is recommended. The standard dilution for peripheral blood is generally 1:2. For bone marrow, a 1:2 or 1:4 dilution are more commonly used. The expectation is that cell recovery should be almost the same.
Helpful?
-
-
Do you have a recommended protocol for purifying PBMCs from larger volumes using Histopaque 1077 in 50 mL tubes instead of 15 mL tubes?
1 answer-
For PBMCs, the catalog numbers for Histopaque 10771, H8889, and 10831 are applicable. The Instructions For Use indicate that a 15 ml centrifuge tube with 3 ml of Histopaque 10771 is recommended. This procedure can also be used for a 50 ml centrifuge tube, considering that the height of both tubes is comparable while their diameter differs. To accommodate a 50 ml centrifuge tube, it is advised to use 15 ml of Histopaque 10771. No alterations are needed for the centrifugation time and washing steps. It is sufficient to wash the isolated cells with isotonic PBS or cell culture media.
Helpful?
-
-
Does Histopaque®-1077 work for mouse blood, or is Ficoll®-Paque Premium 1.084 a better choice?
1 answer-
While Histopaque 10771 can be used, it is important to note that quality control testing for Histopaque 10771 does not include testing with mouse blood. The preferred products for isolating mononuclear cells from rat and mouse blood are Ficoll Paque Premium 1084 (GE17-5446-02) or Histopaque 10831. These products are specifically formulated for rats, mice, and other small mammals for the higher percentage of cell recovery.
Helpful?
-
-
What is the efficacy and reliability of using Histopaque®-1077 for separating low-density neutrophils (LDN) and normal-density neutrophils (NDN) from high-density neutrophils?
1 answer-
The Histopaque 1077 procedure is not designed to purify low-density or high-density neutrophils. Instead, Histopaque 10771 is intended for purifying Peripheral Blood Mononuclear Cells (PBMCs). When using Histopaque 10771, it is expected that the collected mononuclear cells would contain 5% or less of neutrophils, likely in the form of low-density neutrophils.
Helpful?
-
-
Are Histopaque 1077 and Ficoll Paque pure products? What is the percentage of polysaccharide or polysucrose in Histopaque 1077 and Ficoll Paque?
1 answer-
Both Histopaque and Ficoll Paque are formulated with a combination of polysucrose 400 and sodium diatrizoate. On average, Histopaque and Ficoll Paque solutions contain approximately 5.7% polysucrose and 9.0% sodium diatrizoate. The final density of these solutions is expected to be around 1.077 g/ml for both products with a typical density variation of +/- 0.001%.
Helpful?
-
-
Can histopaque-1077 be used to isolate live tumor cells in suspension from dead cells/cell debris?
1 answer-
Histopaque 10771 is primarily intended for isolating mononuclear cells from human blood and bone marrow. It is also utilized in cell culture to eliminate dying cells from hybridomas. However, the effectiveness of Histopaque 10771 or Histopaque 10831 in purifying live cells from deceased liver cells remains untested. Percoll and OptiPrep are common alternative methods.
Helpful?
-