05-656

Anti-IGF-IR Antibody, clone JBW902

clone JBW902, Upstate^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: JBW902, monoclonal
• Application: IHC, IP, WB
• Citations: 17

Propiedades

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• clone: JBW902, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_000875
• UniProt accession no.: P08069
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... IGF1R(3480)

Descripción

General description

95 kDa

There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

Immunogen

GST fusion protein corresponding to residues 1264-1367 of human IGF-1 Receptor

Application

Anti-IGF-IR Antibody, clone JBW902 detects level of IGF-IR & has been published & validated for use in IH, IP & WB.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Biochem/physiol Actions

Recognizes IGF-1 receptor b chain; does not cross react with insulin receptor.

Physical form

Format: Purified

Protein A Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.14 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Protein A purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
C4 cell lysate, HepG2 cells

routinely evaluated by immunoblot on RIPA lysates from C4 cells (mouse 3T3 cells that have been transfected with the human IGF-1 Receptor)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable