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  • Biodegradable and biocompatible poly(ethylene glycol)-based hydrogel films for the regeneration of corneal endothelium.

Biodegradable and biocompatible poly(ethylene glycol)-based hydrogel films for the regeneration of corneal endothelium.

Advanced healthcare materials (2014-03-22)
Berkay Ozcelik, Karl D Brown, Anton Blencowe, Katharina Ladewig, Geoffrey W Stevens, Jean-Pierre Y Scheerlinck, Keren Abberton, Mark Daniell, Greg G Qiao
ABSTRACT

Corneal endothelial cells (CECs) are responsible for maintaining the transparency of the human cornea. Loss of CECs results in blindness, requiring corneal transplantation. In this study, fabrication of biocompatible and biodegradable poly(ethylene glycol) (PEG)-based hydrogel films (PHFs) for the regeneration and transplantation of CECs is described. The 50-μm thin hydrogel films have similar or greater tensile strengths to human corneal tissue. Light transmission studies reveal that the films are >98% optically transparent, while in vitro degradation studies demonstrate their biodegradation characteristics. Cell culture studies demonstrate the regeneration of sheep corneal endothelium on the PHFs. Although sheep CECs do not regenerate in vivo, these cells proliferate on the films with natural morphology and become 100% confluent within 7 d. Implantation of the PHFs into live sheep corneas demonstrates the robustness of the films for surgical purposes. Regular slit lamp examinations and histology of the cornea after 28 d following surgery reveal minimal inflammatory responses and no toxicity, indicating that the films are benign. The results of this study suggest that PHFs are excellent candidates as platforms for the regeneration and transplantation of CECs as a result of their favorable biocompatibility, degradability, mechanical, and optical properties.

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o-Dianisidine dihydrochloride, Suitable for use in glucose determination
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Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
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Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
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OR14608-1G1 g
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OR14608-5G5 g
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CHF 273.20
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Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
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OR14608-1G1 g
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OR14608-5G5 g
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CHF 273.20
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o-Dianisidine dihydrochloride, ≥95%
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OR14608-1G1 g
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CHF 273.20
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Trypan Blue, powder, BioReagent, suitable for cell culture
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o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet
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trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile, matrix substance for MALDI-MS, ≥99.0% (HPLC)
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Trypan Blue, Dye content 60 %, ≥80% (HPLC)
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trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile, ≥98%
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OR14608-1G1 g
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Trypan Blue solution, 0.4%, liquid, sterile-filtered, suitable for cell culture
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Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
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OR14608-1G1 g
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Glucose (GO) Assay Kit, sufficient for 20 assays
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Fluorescein 5(6)-isothiocyanate, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
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OR14608-1G1 g
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Trypan Blue solution, 0.4%, for microscopy
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L-Glutamine, ReagentPlus®, ≥99% (HPLC)
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Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
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OR14608-1G1 g
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Ethylenediaminetetraacetic acid, ≥98.0% (KT)
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L-Glutamine
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Ethylenediaminetetraacetic acid, SAJ special grade, ≥99.0%
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Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
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Ethylenediaminetetraacetic acid disodium salt solution, BioUltra, for molecular biology, pH 8.0, ~0.5 M in H2O
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Ethylenediaminetetraacetic acid, anhydrous, BioUltra, ≥99% (titration)
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Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder
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OR14608-1G1 g
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L-Glutamine, BioUltra, ≥99.5% (NT)
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OR14608-1G1 g
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Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT)
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L-Glutamine, Pharmaceutical Secondary Standard; Certified Reference Material
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Ethylenediaminetetraacetic acid, 99.995% trace metals basis
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Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder
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CHF 273.20