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E6758

Sigma-Aldrich

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

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About This Item

Linear Formula:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS Number:
60-00-4
Molecular Weight:
292.24
Beilstein/REAXYS Number:
1716295
EC Number:
200-449-4
MDL number:
MFCD00003541
UNSPSC Code:
12161700
eCl@ss:
39030907
PubChem Substance ID:
24894634
NACRES:
NA.25

grade

anhydrous

product line

BioReagent

assay

≥98.5%

form

crystalline

reaction suitability

reagent type: chelator

technique(s)

cell culture | mammalian: suitable

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

3  M NaOH: 100 mg/mL

density

1.46 g/cm3 at 20 °C

cation traces

C: 40.7-41.5%
N: 9.3-9.8%

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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Application

Ethylenediaminetetraacetic acid has been used:
  • as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation
  • as a medium component in the hepatic stellate cell (HSCs) culture
  • as a component of homogenization buffer for protein extraction from eye tissues
  • for stopping benzonase reaction in lysis buffer

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Lechuga A, et al.
Bio-protocol, 8(1), 1571-1571 (2018)
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Dai J and Luftig MA
Journal of Immunology, ji1701473-ji1701473 (2018)
The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells
Qiu S, et al.
Biomedicine and Pharmacotherapy, 109, 1841-1850 (2019)
Protein Expressions of the Small GTPase Rho Proteins in Pterygial Tissue and Leukocytes of Patients with Pterygium
Saracaloglu A, et al.
Proceedings, 2(25), 1571-1571 (2018)
Comparison of the efficacy of ethylenediaminetetraacetic acid and tetracycline hydrochloride as root conditioning agents: An in vitro study
Nanda T, et al.
Journal of the International Clinical Dental Research Organization, 4(1), 2-2 (2012)
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Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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