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Lung Section Staining and Microscopy.

Bio-protocol (2017-11-25)
Xiaofeng Zhou, Bethany B Moore
PMID29170747
ABSTRACT

Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson's trichrome staining on lung sections.

MATERIALS
Product Number
Brand
Product Description
E4009
Sigma-Aldrich
Eosin Y, Dye content ~99 %
Pricing and availability is not currently available.
320331
Sigma-Aldrich
Hydrochloric acid, ACS reagent, 37%
Pricing and availability is not currently available.
237086
Sigma-Aldrich
Aluminum potassium sulfate dodecahydrate, ACS reagent, ≥98%
Pricing and availability is not currently available.
A6283
Sigma-Aldrich
Acetic acid, glacial, ReagentPlus®, ≥99%
Pricing and availability is not currently available.
157740
Sigma-Aldrich
Iron(III) chloride, reagent grade, 97%
Pricing and availability is not currently available.
251275
Sigma-Aldrich
Citric acid, ACS reagent, ≥99.5%
Pricing and availability is not currently available.
S8282
Sigma-Aldrich
Sodium phosphate monobasic, BioXtra, ≥99.0%
Pricing and availability is not currently available.
H3136
Sigma-Aldrich
Hematoxylin, certified by the BSC
Pricing and availability is not currently available.