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G005961

HPLC Separation of an Oligonucleotide and Impurities on Proteomix® SAX-NP5 versus Porous SAX

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)
mobile phase: (A) 0.025 M Tris buffer pH9; (B) A + 1.0 M LiCi
gradient: 30 to 75% B in 45 min
flow rate: 1.0 mL/min
detector: UV, 260 nm
injection: 50 μL
sample: 50-mer Oligo

suitability

application for HPLC

application(s)

forensics and toxicology
life science and biopharma

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