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G005945

HPLC Analysis of mAb-X22 on Antibodix® WCX-NP10, Impact of Mobile Phase pH

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)
mobile phase: (A) 10 mM sodium phosphate buffer at 7.5 pH; (B), A + 100 mM NaCl at 7.0 pH
gradient: 15 to 65% B in 60 min
flow rate: 0.8 mL/min
column temp.: 25 °C
detector: UV, 214 nm
injection: 10 μL
sample: MAb-X22, 1.5 mg/mL

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

Legal Information

Antibodix is a registered trademark of Sepax Technologies

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