G005908

HPLC Analysis of Aflatoxins on Ascentis^® Express C18

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U); mobile phase: [A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid; flow rate: 0.400 mL/min; pressure: 4568 psi (315 bar); column temp.: 35 °C; detector: FLD excitation = 360 nm, emission = 440 nm; injection: 20 μL; sample: 200 μL spiked 84:16 acetonitrile:di water in 880 μL di water, Spike concentrations: B1 = 16 ppb; B2 = 4 ppb; G1 = 16 ppb; B2 = 4 ppb
• suitability: application for HPLC
• application(s): food and beverages

Description

Analysis Note

Aflatoxins are a complex of closely related toxins produced as secondary metablolites of fungi. They are found in grains and nuts such as corn, wheat, or peanuts. They are produced by aspergillus flavus and a. Parasiticus. Members of the group include aflatoxin b1, aflatoxin b2, aflatoxin g1, aflatoxin g2, aflatoxin m1, and aflatoxin m2.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Aflatoxin G₂
Aflatoxin G₁
Aflatoxin B₂
Aflatoxin B₁