G005724
HPLC Analysis of Proteins on BIOshell A160 Peptide C18
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)
mobile phase: [A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile
gradient: held at 0% B for 0.5 min; 0 to 100% B in 7 min
flow rate: 0.3 mL/min
pressure: 4743 psi (327 bar)
column temp.: 35 °C
detector: UV, 220 nm
injection: 1 μL
sample: 0.2 - 0.6 g/L in mobile phase A
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
Analyte
Standard
analytical column
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