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G005411

HPLC Analysis of Epinephrine, Epinastine, and Benzalkonium Chlorides on Ascentis® Express HILIC

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 50 mM ammonium acetate; [B] acetonitrile; (10:90, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: ESI(+), XIC m/z 304 & 332 (BAC), 250 (epinastine), 180 (epinephrine)
injection: 10 μL
sample: 10 mg/L ea. in acetonitrile

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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