G005409
HPLC Analysis of Steroids on Ascentis® Express F5, Acetonitrile Mobile Phase
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water; [B] acetonitrile; (70:30, A:B)
flow rate: 0.2 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 2 μL
sample: 50 mg/L in 75:25, water:methanol
suitability
application for HPLC
application(s)
clinical
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analytical column
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