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713-1043

HPLC Analysis of Metabolites of 7,12-Dimethylbenz[a]anthracene on SUPELCOSIL LC-18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)
mobile phase: [A] methanol:[B] water, (50:50, A:B), 10 min to 100:0 at 2.5% /min
flow rate: 0.8 mL/min
detector: fluorescence
sample: rat liver, 9000 × g supernatant fraction from Aroclor-treated animals

suitability

application for HPLC

application(s)

environmental
forensics and toxicology

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Description

    7,12-Dimethylbenz[a]anthracene

Analytical column


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