569232-U

Discovery^® C18 (5 µm) HPLC Columns

L × I.D. 12.5 cm × 4.6 mm, HPLC Column

• Synonym(s): Discovery RP18 HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Discovery^® C18 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4.6 mm
• material: stainless steel column
• agency: suitable for USP L1
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 12% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 12.5 cm × 4.6 mm
• surface area: 300 m²/g
• surface coverage: 3 μmol/m²
• impurities: <10 ppm metals
• matrix: silica gel, high purity, spherical particle platform; fully porous particle
• matrix active group: C18 (octadecyl) phase
• particle size: 5 μm
• pore size: 180 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Application

• Rat Pharmacokinetics and In Vitro Metabolite Identification of KM-819, a Parkinson′s Disease Candidate, Using LC-MS/MS and LC-HRMS.: This study underscores the utility of theDiscovery^® C18 HPLC Column in pharmacokinetic profiling and metabolite identification of a novel therapeutic agent, demonstrating its critical role in the development of Parkinson′s disease treatments (Choi et al., 2024).


• Determination of 2,4-dimethylhydroxybenzene by chromatographic methods in forensic toxicological research of biological material.: Highlights the application of the Discovery^® C18 HPLC Column in forensic toxicology, enhancing the detection and quantification of toxic substances within biological samples, vital for legal and medical investigations (Chernova et al., 2024).


• High-throughput microfluidic chip with silica gel-C18 channels for cyclotide separation.: Illustrates the integration of the Discovery^® C18 HPLC Column in a microfluidic setting for efficient and high-throughput separation of cyclotides, offering advancements in peptide and protein analysis (Ebrahimi et al., 2023).


• Priestia megaterium Metabolism: Isolation, Identification of Naringenin Analogues and Genes Elevated Associated with Nanoparticle Intervention.: This research utilizes the Discovery^® C18 HPLC Column for the advanced study of microbial metabolism under nanoparticle intervention, pushing the boundaries of biotechnological and pharmaceutical research (Al-Theyab et al., 2023).


• A practical strategy enabling more reliable identification of ginsenosides from Panax quinquefolius flower by dimension-enhanced liquid chromatography/mass spectrometry and quantitative structure-retention relationship-based retention behavior prediction.: Demonstrates the versatility of the Discovery^® C18 HPLC Column in complex botanical analyses, particularly in enhancing the reliability of ginsenoside identification, crucial for the development of herbal medicinal products (Sun et al., 2023).

Features and Benefits

• Excellent reproducibility
• Exceptional peak shape for basic and acidic analytes
• Stable, low-bleed LC-MS separations
• Separation of peptides and small proteins
• Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany