The capacity of this 3mL SPE tube, containing 500mg of packing, is 25-100mg. The adsorption capacity and yield vary widely, depending on the analyte(s) and sample matrix.
More information about analyte adsorption capacity can be found on page four of the SPE brochure, please see the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/217/318/solid-phase-extraction-products-brochure-br3075en-ms.pdf