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33027

Supelco

BSTFA, Derivatization Grade

for GC derivatization

Synonym(s):

N,O-Bis(trimethylsilyl)trifluoroacetamide

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About This Item

CAS Number:
25561-30-2
EC Number:
247-103-9
UNSPSC Code:
41116105

grade

for GC derivatization

reaction suitability

reagent type: derivatization reagent
reaction type: Acylations
reagent type: derivatization reagent
reaction type: Silylations

packaging

pkg of 25 mL

technique(s)

GC/MS: suitable

InChI

1S/C8H18F3NOSi2/c1-14(2,3)12-7(8(9,10)11)13-15(4,5)6/h1-6H3

InChI key

XCOBLONWWXQEBS-UHFFFAOYSA-N

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General description

BSTFA is a derivatizing agent widely used in the derivatization of estrogenic steroids, thereby resulting in the formation of trimethylsilyl (TMS) derivative. It is also known to react with the aromatic hydroxyl group.[1]

Application

BSTFA was used for silylation of samples during GC-MS analysis of Glutathione conjugates of Prostaglandin A1.[2]
Suitable for the derivatization of Υ-aminobutyric acid, n-acetylneuraminic acid, arginine, dipeptides, glycine, glycosphingolipi, n-hydroxylamines, lysine, nitrilotriacetate, phenols (sterically hindered), phospholipids, phosphoserine and phosphothreonine, prostaglandins F2α, prostaglandin H2 methyl ester, prostaglandins F2α, sulfur amino acids, terephthalate prepolymer mixture, thiohydantoins of amino acids, and thromboxane B2.

Other Notes

Reagent for N-acetyl, O-trimethylesilyl, trimethylsilyl, trimethylsilyl diglyceride, trimethylsilyl ether, sulfonyl trimethylsilyl esters and trimethylsilyl thiohydantoins.

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pictograms

FlameExclamation mark
GHS02,GHS07

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Warning

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

93.2 °F

flash_point_c

34 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
LC26066V
LC24876V
LC23392V
LC22715V
LC22492V

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N.E. Hoffman and K.A. Peteranetz
Analytical Letters, 5, 589a-589a (1972)
L M Cagen et al.
The Journal of biological chemistry, 251(21), 6550-6554 (1976-11-10)
When prostaglandin A1 was incubated with a Tris/saline suspension of washed human red blood cells, a substantial amount was converted to polar metabolites. These were purified by solvent extraction, XAD-2 column, and cellulose thin layer chromatography and characterized by chromatography
E.R.Atkinson and S.I. Calouche
Analytical Chemistry, 43, 460-460 (1971)
C.W. Gehrke and K. Leimer
Journal of Chromatography A, 57, 219-219 (1971)
B. Wengle and K.Hellstrom
Scandinavian Journal of Clinical and Laboratory Investigation, 28, 477-477 (1977)
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