ZRB2769

Anti-Interleukin-21 Antibody, clone 2B13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): IL-21, Za11
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 2B13 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 18.65 kDa; observed mol wt ~17 kDa
• purified by: using Protein A
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_068575
• UniProt accession no.: Q9HBE4
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... IL21(59067)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2B12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Interleukin-21. It targets an epitope within 23 amino acids from the C-terminal half.

Immunogen

KLH-conjugate linear peptide corresponding to 23 amino acids from the C-terminal half of human Interleukin-21.

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Interleukin 21 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Interleukin-21 in lysates from HepG2 and, NIH3T3 cells and and Rat spleen tissue.

Flow Cytometry Analysis: 1 µg from a representative lot detected Interleukin-21 in one million Jurkat cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Interleukin-21 in Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Interleukin-21 in Human spleen tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Interleukin-21 (UniProt Q9HBE4; also known as IL-21, Za11) is encoded by the IL21 gene (Gene ID 59067) in human. IL-21 is a cytokine with immunoregulatory activity and promotes the transition between innate and adaptive immunity. It is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to produce the mature protein. It is considered as a pleiotropic cytokine with actions on a broad range of lymphoid, myeloid, and epithelial cells and has effect on cell proliferation, survival, differentiation, and function. It plays a key role in B cell differentiation to plasma cells and in the development of T follicular helper cells. It promotes functional germinal centers and immunoglobulin production. Together with IL-6, it controls the early generation of Tfh cells and is critical for an effective antibody response to acute viral infection. IL-21 also has a key role in the development of T helper 17 (TH17) cells, which contribute to pathogenesis in a range of inflammatory diseases. IL-21 signaling is mediated via heterodimers of the IL-21 receptor (IL-21R) and the common cytokine receptor -chain, c. Mutations in IL21 gene have been linked to Immunodeficiency, common variable, 11 (CVID11) that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. Two isoforms of IL-21 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.(Ref.: Spolski, R., and Leonard, WJ. (2014). Nat. Rev, Drug Dis. 13(5); 379-395; J Parrish-Novak, J., et al. (2000). Nature. 408(6808); 57-63).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable