ZRB2318

Anti-p-LATS1 (Thr1079) Antibody, clone 2D2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): EC:2.7.11.1, Large tumor suppressor homolog 1, Serine/threonine-protein kinase LATS1, WARTS protein kinase (h-warts)
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2D2, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 126.87 kDa; observed mol wt ~150 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): rat, mouse
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_004681
• UniProt accession no.: O95835
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... LATS1(9113)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2D2 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects LATS1 phosphorylated on Threonine 1079. It targets an epitope within 11 amino acids surrounding phosphothreonine from the C-terminal region. This antibody is predicted to cross react with LATS2 when phosphorylated at site Thr1041.

Immunogen

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphothreonine 1079 in human Serine/threonine-protein kinase LATS1.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells treated with Calyculin/Okadaic Acid cells.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected LATS1 phosphorylated on Threonine 1079 in lysate from overnight serum starved HeLa cells treated for 30 minutes with Calyculin A (50 nM) and Okadaic Acid (500 nM).

Tested Applications

Immunocytochemistry Analysis: A 1:1,00 dilution from a representative lot detected p-LATS1(Thr1079) in HeLa cells treated with Calyculin A (100 nM for 30 minutes).

Peptide Inhibition Assay: Target band detection in lysate from overnight serum starved HeLa cells treated for 30 minutes with Calyculin A (50 nM) and Okadaic Acid (500 nM) was prevented by pre-blocking of a representative lot with the immunogen phospho-peptide, but not the corresponding non-phospho peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Serine/threonine-protein kinase LATS1 (UniProt: O95835; also known as EC: 2.7.11.1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts) is encoded by the LATS1 (also known as WARTS) gene (Gene ID: 9113) in human. Serine/threonine-protein kinase LATS1 serves as a negative regulator of YAP1 in the Hippo signaling pathway, which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. It is shown to be phosphorylated by STK3/MST2 at Serine 909 and Threonine 1079, which results in its activation. However, phosphorylation at Serine 464 by NUAK1 and NUAK2 LEADS to diminished protein level and it is essential for regulation of cellular senescence and cellular ploidy. YAP1 phosphorylation by LATS1 inhibits its translocation into the nucleus to regulate cellular genes that are important for cell proliferation, cell death, and cell migration. LATS1 is shown to be expressed in all adult tissues except in lung and kidney. It localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis. LATS1 negatively regulate G2/M transition by down-regulating CDK1 kinase activity. Blocking of LATS1 and 2 is reported to promote the luminal phenotype and increases the number of bipotent and luminal progenitor cells in most human breast cancers. Absence of LATS stabilizes ER and the Hippo effectors YAP/TAZ, which control breast cell fate via intrinsic and paracrine mechanisms. LATS1 acts as a tumor suppressor in cervical cancer by regulating the expression of cyclin E, p27, matrix metalloproteinase 9 (MMP9) and yes-associated protein 1 (YAP). Overexpression of LATS2 leads to the development of acute myeloid leukemia (AML). Therefore, it is considered as a potential therapeutic target for AML treatment. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, Affinity , reproducibility, and stability over conventional monoclonals. (Ref.: Britschgi, A., et al. (2017). Nature. 541(7638); 541-545; Hao, Y., et al. (2008). J. Biol. Chem. 283(9); 5496-5509).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable