ZRB1973

Anti-Ubiquityl H2B-Lys120 Antibody, clone 1F17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Histone H2B type 1-B;H2B-clustered histone 3;Histone H2B.1;Histone H2B.f; H2B/f
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F17, recombinant monoclonal
• description: 1F17 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 13.95 kDa; observed mol wt ~24 kDa
• purified by: using Protein A
• species reactivity: rat, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_066406
• UniProt accession no.: P33778
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: ubiquitination (Lys120)
• Gene Information: human ... H2BC3(3018)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Histone H2B ubiquitylated on lysine 120.

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids surrounding ubiquitylated lysine 120 of human Histone 2B.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell acid extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ubiquityl Histone H2B-Lys120 in HeLa cell acid extract.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ubiquityl Histone H2B-Lys120 in HL-60 cell and Rat2 cell acid extracts .

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ubiquityl Histone H2B-Lys120 in human liver tissue sections.

Peptide Inhibition Assay: Target band detection in a lysate from HeLa cell acid extract was prevented by pre-blocking of a representative lot with Ubiquityl Histone H2B-Lys120 peptide, but not the corresponding non- Ubiquityl H2B peptide.

Affinity Binding Assay: A representative lot of this antibody bound Ubiquityl Histone H2B-Lys120 peptide with a KD of 3.5 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Histone H2B type 1-B (UniProt: P33778; also known as H2B-clustered histone 3, Histone H2B.1, Histone H2B.f (H2B/f) is encoded by the H2BC3 (also known as H2BFF, HIST1H2BB) gene (Gene ID: 3018) in human. Histone H2B is a core component of nucleosome that wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B features a main globular domain and a long N terminal tail. Histone H2B mono-ubiquitinated on lysine120 during transcriptional activation by RAD6E2 protein and is associated with the transcribed regions of highly expressed genes. It is a key histone modification that plays critical roles in gene transcriptional regulation and higher order chromatin organization. Mono-ubiquitination of histone H2B stimulates transcriptional elongation by facilitating FACT-dependent chromatin remodeling. De-ubiquitination of histone H2B lysine120 by USP22 is a required step in transcriptional activation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, S., et al. (2012). Cell Research. 22(9); 1402-1405; Zhu, B., et al. (2006). Mol. Cell. 20(4); 601-611).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable