ZRB1878

Anti-AP-2α Antibody, clone 1N19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): AP-2 transcription factor, AP2TF, Activating enhancer-binding protein 2-alpha, Activator protein 2 (AP-2), TFAP2, Transcription factor AP-2-alpha
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1N19, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 48.06 kDa; observed mol wt ~48 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_003211
• UniProt accession no.: P05549
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... TFAP2A(7020)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1N19 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects AP-2 . It targets an epitope within 15 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human AP-2 .

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AP-2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AP-2 in lysate from NIH/3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected AP-2 in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected AP-2 in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound with AP-2 peptide at least ten thousand-fold (10,000X) higher than with the non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected AP-2 in NIH/3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Transcription factor AP-2-alpha (AP2a) (UniProt: P05549; also known as AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2 (AP-2)) is encoded by the TFAP2A (also known as AP2TF, TFAP2) gene (Gene ID: 7020) in human. Transcription factor AP-2-alpha (AP-2 ) is a member of the AP-2 family of transcription factors that consists of five different proteins in humans. It is composed of several functional domains, including a proline/glutamine-rich transactivation domain. The helix-span-helix motif, together with a central basic region, mediates dimerization and DNA binding. AP-2 may act as either a homodimer or heterodimer with paralogs from its family and regulates genes involved in cell proliferation, apoptosis, and differentiation by binding to specific promoter regions and modulating their transcription. AP-2 also plays a pivotal role in the regulation of gene expression during embryonic development. It is involved in the differentiation and development of neural crest cells, the formation of the face, limbs, and the central nervous system. In adults, its expression is more restricted but can be found in certain tissues such as the skin, breast, and parts of the nervous system. The expression of TFAP2A is tightly regulated and varies depending on the developmental stage and tissue type. Mutations and dysregulation of TFAP2A are associated with several developmental disorders and diseases. Mutations in the TFAP2A gene can lead to branchio-oculo-facial syndrome, a rare congenital disorder characterized by craniofacial, ocular, and branchial defects. In cancer, altered expression of TFAP2A has been implicated in the progression and metastasis of various types, including breast cancer, melanoma, and glioblastoma. TFAP2A can act as either a tumor suppressor or an oncogene, depending on the cellular context and the genes it regulates. (Ref.: Eckert, D., et al. (2005). Genome Biol., 6(13), Article 246; Kolat, D., et al, (2019) Biosci. Rep. 39 (3): BSR20181928.) This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable