ZRB1821

Anti-SLC12A3 Antibody, clone 3I12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Solute carrier family 12 member 3;Na-Cl cotransporter;NCC;Na-Cl symporter;Thiazide-sensitive sodium-chloride cotransporter
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3I12, recombinant monoclonal
• description: 3I12 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 113.14 kDa; observed mol wt ~130 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001119580.1
• UniProt accession no.: P55017
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... SLC12A3(6559)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3I12 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Solute carrier family 12 member 3 (SLC12A3). It targets an epitope within 16 amino acids from the N-terminal, cytoplalsmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal, cytoplasmic domain of human Solute carrier family 12 member 3 (SLC12A3).

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse kidney tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SLC12A3 in Mouse kidney tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected His-tagged Human SLC12A3 recombinant fragment.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC12A3 in HEK293 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SLC12A3 in human kidney and mouse kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound SLC12A3 peptide with a KD of 1.4 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Solute carrier family 12 member 3 (UniProt: P55017; also known as Na-Cl cotransporter, NCC, Na-Cl symporter, Thiazide-sensitive sodium-chloride cotransporter) is encoded by the SLC12A3 (also known as NCC, TSC) gene (Gene ID: 6559) in human. SLC12A3 is a multi-pass membrane protein with seven cytoplasmic domains and twelve transmembrane domains. It is an electroneutral sodium and chloride ion cotransporter responsible for maintaining electrolyte homeostasis via mediating the reabsorption of these ions. It is predominantly expressed in the kidney and is localized in the distal convoluted tubules. SLC12A3 is a target of thiazide diuretics that are used in the treatment of hypertension. Three isoforms of SLC12A3 have been described that are produced by alternative splicing. Mutations in SLC12A3 gene are known to cause Gitelman syndrome, an autosomal recessive disorder that is characterized by hypokalemic alkalosis in combination with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, N., and Gu, HF. (2022). Front. Genet. 13; 799224; Riviera-Munoz, E., et al. (2007). J. Am. Soc. Nephrol. 18 (4); 1271-1283).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable