ZMS1119
Anti-SV40 T Antigen Antibody, clone PAb416 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Anti-XLF/NHEJ1 clone 1C16 ZooMAb® Rabbit Monoclonal, LT, LT-AG, Large T antigen
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
PAb416, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 81.62 kDa
observed mol wt ~80 kDa
purified by
using protein G
species reactivity
virus
species reactivity (predicted by homology)
all
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
vaccinia virus ... SV40gp6(29031019)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone PAb416 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Large T antigen of Simian virus 40.
Immunogen
Purified large T-antigen of SV40
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from SV40 infected COS-1 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Large T antigen in lysate from SV40 infected COS-1 cells.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SV40 Large T Antigen in COS-7 cells.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected SV40 Large T Antigen in one million COS-7 Cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in lysate from SV40 infected COS-1 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Large T antigen in lysate from SV40 infected COS-1 cells.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SV40 Large T Antigen in COS-7 cells.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected SV40 Large T Antigen in one million COS-7 Cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Large T antigen (UniProt: P03070; also known as LT, LT-AG) is encoded by the SV40gp6 gene (Gene ID: 29031019) in Simian virus 40. Large T antigen is a key early protein that is reported to be essential for both driving viral replication and inducing cellular transformation. It plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. It displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. It is shown to participates in the modulation of cellular gene expression preceding viral DNA replication and this step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. It is also known to induce the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. It inhibits host TP
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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