Z364010
Practical Guide to Membrane Protein Purification
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About This Item
ISBN-10:
0-12-725550-8
ISBN-13:
978-0-12-725550-7
UNSPSC Code:
55101509
publication info
G. Von Jagow and H. Schagge, ed., Academic Press, 1994, 162 pp., soft cover
manufacturer/tradename
Elsevier
General description
Written for those who have some familiarity with separation of water-soluble proteins, but who may not be aware of the problems involved in the isolation of membrane proteins. This book is an essential manual for investigations of structure and function of native membrane proteins.
Table of Contents
Strategies and Techniques: Purification Strategies for Membrane Proteins.
Chromatographic Techniques and Basic Operations in Membrane Protein Purification.
Denaturing Electrophoretic Techniques.
Native Gel Electrophresis.
Discussion of Selected Isolation Protocols: Isolation of Complex III, of Subcomplexes, and of Protein Subunits from Bovine Heart: Triton X–100/Hydroxylapatite.
Chromatographic Purification of F−1F−0–ATPase and Complex I from Bovine Heart Mitochondria: FPLC–Anion Exchange and Dye–Ligand Chromatography.
Isolation of Ubiquino l: Cytochrome c Oxidoreductase (Complex III) from Fungal and Plant Mitochondria: Affinity Chromatography.
Isolation of Wolinella succinogenes Hydrogenase: Chromatofocusing.
Photosystem I From Cyanobacteria Isolated in Crystallizing Form by Pre
Chromatographic Techniques and Basic Operations in Membrane Protein Purification.
Denaturing Electrophoretic Techniques.
Native Gel Electrophresis.
Discussion of Selected Isolation Protocols: Isolation of Complex III, of Subcomplexes, and of Protein Subunits from Bovine Heart: Triton X–100/Hydroxylapatite.
Chromatographic Purification of F−1F−0–ATPase and Complex I from Bovine Heart Mitochondria: FPLC–Anion Exchange and Dye–Ligand Chromatography.
Isolation of Ubiquino l: Cytochrome c Oxidoreductase (Complex III) from Fungal and Plant Mitochondria: Affinity Chromatography.
Isolation of Wolinella succinogenes Hydrogenase: Chromatofocusing.
Photosystem I From Cyanobacteria Isolated in Crystallizing Form by Pre
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