XNAT2R
Extract-N-Amp™ Tissue PCR Kit
sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonym(s):
Tissue direct PCR kit
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About This Item
UNSPSC Code:
41106303
NACRES:
NA.55
Recommended Products
usage
sufficient for 1000 amplifications
sufficient for 1000 extractions
sufficient for 1000 reactions
feature
dNTPs included
hotstart
technique(s)
PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
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General description
The Extract-N-Amp™ Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by direct PCR (polymerase chain reaction) . A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract.
Application
Extract-N-Amp™ tissue PCR kit has been used for DNA extraction and polymerase chain reaction (PCR).
Features and Benefits
- Fast – rapid extraction of genomic DNA for PCR in 15 minutes
- Convenient – no long enzymatic digestions or column purification required
- Practical – perfect for quick genomic DNA isolation for genotyping
- Flexible – protocols available for mouse-tails, hair, animal tissue, saliva, and buccal swabs
- Specific – Hot start antibody for highly specific PCR amplification of genomic DNA
- Extract stable at 4 oC for at least 6 months
Components
Kits contain Tissue Preparation Solution, Extraction Solution, and Extract-N-Amp PCR Reaction Mix.
Principle
The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
This kit includes a clear solution of PCR readymix. To order the REDExtract-N-Amp Tissue PCR Kits with the inert tracking and loading dye in the PCR readymix, please order XNAT-1000RXN"
This kit includes a clear solution of PCR readymix. To order the REDExtract-N-Amp Tissue PCR Kits with the inert tracking and loading dye in the PCR readymix, please order XNAT-1000RXN"
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
signalword
Danger
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Protocols
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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