X100RS

Triton^™ X-100 reduced

• Synonym(s): Polyoxyethylene (10) isooctylcyclohexyl ether
• Linear Formula: 4-(C₈H₁₇)C₆H₁₀(OCH₂CH₂)_nOH, n~10
• CAS Number: 92046-34-9
• MDL number: MFCD00167048
• UNSPSC Code: 12161900
• NACRES: NA.25

Properties

• biological source: synthetic (oragnic)
• Quality Level: 100
• description: non-ionic
• form: viscous liquid
• mol wt: micellar average mol wt 80,000; average mol wt 625
• aggregation number: 100-155
• refractive index: n20/D 1.473 (lit.)
• CMC: 0.2-0.9
• mp: 48 -50 °C (118 - 122 °F)
• transition temp: cloud point 65 °C
• solubility: water: soluble, clear to slightly hazy, colorless
• density: 1.029 g/mL at 25 °C (lit.)
• absorption: ≤0.25 at 277 nm in H₂O at 0.5%
• HLB: 13.5
• SMILES string: CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO
• InChI: 1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3
• InChI key: QQJNBKDKLMCALZ-UHFFFAOYSA-N

Description

General description

Triton^™ X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a vital component in lysis buffers. Widely utilized in biochemical and cell biology research, Triton^™ X-100 facilitates the isolation of lipid rafts, enhancing the solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, this product exhibits excellent wetting properties and emulsification enhancement.

In cell biology, Triton^™ X-100 plays a crucial role in solubilizing membrane-bound proteins, preserving their native conformation in solution. Furthermore, Triton^™ X-100 reduced, resulting from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative, has been associated with potential benefits in enzyme digestion enhancement and effects on the photoisomerization of bacteriorhodopsin. The versatility of Triton^™ X-100 in various research applications underscores its significance as a fundamental tool in biochemical and cell biology investigations.

Application

Triton^™ X-100 reduced has been used:

• in nuclear magnetic resonance (NMR) sample tube for lysing/rupturing the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles
• in cell permeabilization
• as a component in Tris/HCl to determine hydrolysis of the esters for enzyme activity assays

Features and Benefits

• Non-ionic surfactant
• Improves solubility and dispersibility of substances
• Excellent wetting properties
• Enhances emulsification
• High purity product for research applications

Preparation Note

Hydrogenated to reduce UV absorbance.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H315,H319,H335,H411
• pcodes: P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 235.4 °F - closed cup
• flash_point_c: 113 °C - closed cup
• ppe: dust mask type N95 (US), Eyeshields, Gloves