T0319

Trizma^® Pre-set crystals

BioPerformance Certified, pH 7.4, average M_w 151.6

• Synonym(s): Tris crystals
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• form: crystalline powder
• mol wt: average M_w 151.6
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 7.12 (37 °C, 0.1 M)(lit.); 7.40 (25 °C, 0.1 M)(lit.); 7.4; 7.97 (5 °C, 0.1 M)(lit.)
• useful pH range: 7.0-9.0
• solubility: deionized water: soluble at 25 °C, clear, colorless (pH 7.4, 1 pouch dissolved in 1 L to yield a 0.1 M Trizma solution)
• application(s): diagnostic assay manufacturing

Description

Application

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Packaging

In convenient foil pouches

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves