SRP5053
NEK9 (1-979), (deleted fragment 346-731), active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
DKFZp434D0935, MGC138306, MGC16714, NEK8, NERCC, NERCC1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
102-138 nmol/min·mg
mol wt
~115 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NEK9(91754)
General description
NEK9 is a member of the NEK family and has high homology to NEK1, NEK3 and NEK4. NEK9 is activated during mitosis and binds specifically to RAN GTPase and is a substrate for CDC2 phosphorylation. NEK9 plays a role in the control of mitotic progression and is regulated by CDC2 and RAN GTPase. Overexpression of both active and inactive variants of NEK9 is toxic to cells and inhibits cell division causing abnormal nuclear morphologies. NEK9 can catalyze the phosphorylation of recombinant NEK6 and NEK7 in vitro leading to its activation. This suggests that NEK9 may be responsible for activation of NEK6 and NEK7 during mitosis.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
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Joan Roig et al.
Genes & development, 16(13), 1640-1658 (2002-07-09)
The protein kinase NIMA is an indispensable pleiotropic regulator of mitotic progression in Aspergillus. Although several mammalian NIMA-like kinases (Neks) are known, none appears to have the broad importance for mitotic regulation attributed to NIMA. Nercc1 is a new NIMA-like
Christopher Belham et al.
The Journal of biological chemistry, 278(37), 34897-34909 (2003-07-04)
The Nek family of protein kinases in humans is composed of 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying
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