SRP4015

CD40Ligand/TRAP from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): CD154 antigen, CD40-L, T cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: <0.1 μg/mL ED₅₀
• mol wt: ~16.4 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: P27548
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Cd40lg(21947)

Description

General description

CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant mouse CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.

Physical form

Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 plus 0.1 M Arginine.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.