SRP0394
EZH2/EED/SUZ12/RbAp48 active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)
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About This Item
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
48 kDa (RbBP5)
51 kDa (EED)
86 kDa (EZH2)
87 kDa (SUZ12)
packaging
pkg of 50 μg
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EED(8726) , EZH2(2146) , RBBP4(5928) , SUZ12(23512)
General description
Complex of human EZH2 (GenBank Accession No. NM_004456), amino acids 2-end with N-terminal His-tag, MW= 86 kDa; human EED (GenBank Accession No. NM_003797) amino acids 2-end with N-terminal Flag-tag, MW= 51 kDa, human SUZ12 (GenBank Accession No. NM_015355) amino acids 2-end with N-terminal His-tag, MW = 87 kDa, and human RbAp48 (GenBank Accession No. NM_005610) amino acids 2-end with N-terminal His-tag, MW = 48 kDa, co-expressed in Baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Journal of Virology, 5, 32-32 (2008)
Science (New York, N.Y.), 322(5908), 1695-1699 (2008-11-15)
Enhancer of zeste homolog 2 (EZH2) is a mammalian histone methyltransferase that contributes to the epigenetic silencing of target genes and regulates the survival and metastasis of cancer cells. EZH2 is overexpressed in aggressive solid tumors by mechanisms that remain
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