SRE0116

Salt Active Nuclease (SAN)

recombinant, expressed in Pichia pastoris, Triton-free

• Synonym(s): endonuclease, recombinant nuclease, salt active endonuclease, salt tolerant nuclease
• UNSPSC Code: 12352204
• NACRES: NA.77

Properties

• recombinant: expressed in Pichia pastoris
• Quality Level: 200
• composition: Triton-free
• packaging: pkg of 25 kU
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

This Salt Active Nuclease (SAN) has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM. It is recombinantly expressed in Pichia pastoris.

Application

• Can be used directly in cell medium or harvested supernatant without buffer adjustments
• Ideal for DNA removal directly in mammalian cell media

Features and Benefits

• Active at physiological conditions
• Endotoxin tested Animal origin free without buffer adjustments.

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl₂, 150 mM NaCl, and 50 μg/ml calf thymus DNA. 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl₂, 150 mM NaCl, and 50 μg/ml calf thymus DNA

Reconstitution

In solution

Other Notes

• Temperature: 25 – 50 °C
• Salt concentration (NaCl): 50 – 400 mM, optimal: 125 – 250 mM
• Mg²⁺: > 0.5 mM is required for activity, optimal: 4 - 15 mM
• pH: 6.5 – 9.5, optimal: 7.2 - 8.7
• Not recommended to use in presence of EDTA or urea.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable