SRE0078

Aprotinin

≥3 TIU/mg solid, lyophilized powder, ≥96% (HPLC), Suitable for manufacturing of diagnostic kits and reagents

• Synonym(s): BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)
• Empirical Formula (Hill Notation): C₂₈₄H₄₃₂N₈₄O₇₉S₇
• CAS Number: 9087-70-1
• Molecular Weight: 6511.44
• EC Number: 232-994-9
• MDL number: MFCD00130541
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• product name: Aprotinin from bovine lung, lyophilized powder, ≥3 TIU/mg solid
• description: For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US.
• Quality Level: 400
• assay: ≥96% (HPLC)
• form: lyophilized powder
• specific activity: ≥3 TIU/mg solid
• mol wt: ~6,500
• shipped in: wet ice
• storage temp.: 2-8°C
• InChI key: ZPNFWUPYTFPOJU-UHFFFAOYSA-N

Description

Application

Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable