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SBR00045

Sigma-Aldrich

Gentamicin-Amphotericin B Ready Made Solution

Gentamicin 25mg/mL and Amphotericin B 125µg/mL

Synonym(s):

Gentamicin-Amphotericin B Ready Made Solution

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About This Item

UNSPSC Code:
51281607
NACRES:
NA.76

Quality Level

specific activity

Antibacterial and antifungal

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
fungi
mycoplasma
yeast

mode of action

cell membrane | interferes
protein synthesis | interferes

Storage temp.

−20°C

General description

Gentamicin-Amphotericin B is an aqueous based stable formulation, intended to eradicate sensitive bacterial and fungal infections. Gentamicin is an aminoglycoside class antibiotic inhibiting the protein synthesis preventing the growth of gram positive and gram-negative bacteria. Amphotericin B is a well-known polyene antifungal compound used in cell culture. The Gentamicin-Amphotericin B mixture is especially usable for cell culture applications and as part of viral transfer media (VTM).

Other Notes

  • The Gentamicin-Amphotericin B mixture is composed from Gentamicin 25mg/ml and Amphotericin B 125 µg/mL.
  • The working concentrations according to CDC SOP# DSR-052-05 are 100 µg/mL for Gentamicin and 0.5 µg/mL for Amphotericin B.
  • 2mL Gentamicin-Amphotericin B stock mixture is sufficient to prepare 500 mL working concentration.
  • The product is packed under aseptic conditions.

Pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Criticality of in-house preparation of viral transport medium in times of shortage during COVID-19 pandemic
Petersen, Jeffrey, Sharvari Dalal, et al
Laboratory Medicine, 52, no. 2, e39-e45 (2021)

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