SAB6010081

Anti-phospho-RAF1 (S621) antibody, clone 1C2, Rabbit Monoclonal

recombinant, expressed in HEK293F cells

• Synonym(s): RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF
• UNSPSC Code: 12352203

Properties

• recombinant: expressed in HEK 293 F cells
• Quality Level: 100
• conjugate: unconjugated
• antibody product type: primary antibodies
• clone: 1C2, recombinant monoclonal
• form: liquid
• mol wt: 150 kDa
• purified by: affinity chromatography
• species reactivity: human
• concentration: 1.5mg/ml
• technique(s): ELISA: 2000:10000; flow cytometry: 1:50-1:200; immunofluorescence: 1:50-1:200; immunohistochemistry: 1:50-1:200
• isotype: IgG
• UniProt accession no.: P04049
• shipped in: wet ice
• storage temp.: -10 to -25°C
• target post-translational modification: phosphorylation (pSer621)

Description

General description

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at ′Ser-75′. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Immunogen

A synthesized peptide derived from human Phospho-RAF1 (S621)

Sequence

SA-pS-EP

Physical form

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage and Stability

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable