SAB6010057

Anti-phospho-ERN1 (S724) antibody, clone 1F12, Rabbit Monoclonal

recombinant, expressed in HEK293F cells

• Synonym(s): Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, ERN1
• UNSPSC Code: 12352203

Properties

• recombinant: expressed in HEK 293 F cells
• Quality Level: 100
• conjugate: unconjugated
• antibody product type: primary antibodies
• clone: 1F12, recombinant monoclonal
• form: liquid
• mol wt: 150 kDa
• purified by: affinity chromatography
• species reactivity: human
• concentration: 0.6mg/ml
• technique(s): ELISA: 2000:10000; flow cytometry: 1:50-1:200; immunofluorescence: 1:50-1:200; immunohistochemistry: 1:50-1:200
• isotype: IgG
• UniProt accession no.: O75460
• shipped in: wet ice
• storage temp.: -10 to -25°C
• target post-translational modification: phosphorylation (pSer724)

Description

General description

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation (PubMed:9637683, PubMed:11175748, PubMed:12637535). The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis (PubMed:24508390).

Immunogen

A synthesized peptide derived from human Phospho-ERN1 (S724)

Sequence

GRH-pS-FSR

Physical form

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage and Stability

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable