SAB4702103

Monoclonal Anti-Neurofilament H, purified produced in mouse

• Synonym(s): CMT2CC, NEFH, NFH, Neurofilament heavy chain
• UNSPSC Code: 12352203

Properties

• conjugate: unconjugated
• Quality Level: 100
• antibody product type: primary antibodies
• clone: NF-01, monoclonal
• form: buffered aqueous solution
• species reactivity: (Mammalian)
• concentration: 1 mg/ml
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P12036
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... NEFH(4744)

Description

General description

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.

Specificity

The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Immunogen

Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.

Physical form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified by protein-A affinity chromatography.

Storage and Stability

Store at 2-8°C. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable