SAB4504207

Anti-phospho-Smad2 (pSer²⁵⁰) antibody produced in rabbit

affinity isolated antibody

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 52 kDa
• species reactivity: mouse, human, rat
• concentration: ~1 mg/mL
• technique(s): ELISA: 1:10000; western blot: 1:500-1:1000
• NCBI accession no.: Q15796
• UniProt accession no.: Q15796
• application(s): research pathology
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer250)
• Gene Information: human ... SMAD2(4087)

Description

General description

SMAD2 (SMAD family member 2) is also called as MADR2 (substrate of the TGFβ receptor). It is located on human chromosome 18q21. This gene belongs to the mothers against Dpp (MAD) related family of proteins. SMAD2 has two highly conserved amino and carboxyl-terminal domains (MH1 and MH2 domains) but has no known structural motifs.

Immunogen

The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Ser250.

Immunogen Range: 216-265

Application

Anti-phospho-Smad2 (pSer250) antibody has been used in immunoblotting.

Biochem/physiol Actions

SMAD2 (SMAD family member 2) plays an important role in TGF-β(transforming growth factor β)/activin signaling pathways. Mutations in SMAD2 (SMAD family member 2) results in arterial aneurysms and dissections. Upregulation of Smad2 blocks the multiplication of gingival epithelial cells. Smad2 positively and negatively controls TGFβ-dependent transcription with the help of forkhead DNA-binding protein FAST2.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable