SAB4200826

Anti- Cholera Toxin-Peroxidase antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Cholera enterotoxin subunit B (ctxB), Cholera enterotoxin, A chain (ctxA)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: peroxidase conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• description: Research area: Microbiome
• form: lyophilized powder
• mol wt: subunit mol wt ~12 kDa (non-toxic CTxB ); subunit mol wt ~27 kDa (the toxic CTxA )
• species reactivity: Vibrio cholerae
• concentration: ~4 mg/mL
• technique(s): direct ELISA: 1:8,000-1:16,000 using 5 μg/ml Cholera Toxin for coating
• UniProt accession no.: P01555 (ctxA); P01556 (ctxB)
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Vibrio cholerae ... ctxA(57740120) , ctxB(57740119)

Description

General description

Cholera toxin (CTx) also known as choleragen, is an enterotoxin produced by the Gram-negative bacterium Vibrio cholerae that naturally habitat in fresh or saltwater environments. CTx is composed of two subunits, the toxic CTxA (~27 kDa) and non-toxic CTxB (~12 kDa) assembled with the stoichiometry AB5. The V. cholerae virulence factors CtxA and CtxB are located at the CTX phage genome integrated within the bacterial chromosome.

Specificity

Anti-Cholera Toxin-peroxidase antibody specifically recognizes Cholera Toxin and has no cross reactivity with Staphylococcal Enterotoxin A (SEA), Staphylococcal Enterotoxin B (SEB) or Pseudomonas Exotoxin A

Immunogen

purified toxin from Vibrio cholera

Application

Anti- Cholera Toxin-Peroxidase antibody produced in rabbit has been used in direct enzyme linked immunosorbent assay.

Biochem/physiol Actions

The A1 fragment of the cholera toxin (CTx)A subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the α-subunit of Gs (Gsa), a regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells. Most of the V. cholerae species do not cause any disease in human, but few including serotypes O1 and O139 can cause cholera pandemics. Antibodies specific for cholera toxin may be used in studies of structural and functional aspects of toxin membrane interactions and for the detection of CTxB, when used, for example, as an adjuvant when injected mucosally together with the desired antigen.

Physical form

Supplied as a lyophilized powder

Storage and Stability

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable