SAB4200778

Anti-NRAS (internal) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-GTPase NRas, Anti-transforming protein N-Ras
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: predicted mol wt ~21 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 2.5-5 μg/mL using human MCF7 cell line extract; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human melanoma sections
• UniProt accession no.: P01111
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NRAS(4893)

Description

General description

NRAS, also known as GTPase NRas, Transforming protein N-Ras, or Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog, is a member of the Ras protein family, together with KRAS (Kirsten RAS), and HRAS (Harvey RAS), comprising a family of low-molecular-weight GTPases. The Ras family is named for a retrovirus that induced rat sarcomas that were later found to have activating RAS mutations. Ras family proteins serve as molecular switches in regulating pathways that are responsible for diverse cellular processes such as proliferation, differentiation, migration, and apoptosis.3 Ras proteins are highly homologous regarding their primary amino acid sequence and the differences among them concentrated in their C-terminal region. Anti-NRAS (internal) antibody produced in rabbit specifically recognizes human NRAS and does not cross-react with KRAS.

Immunogen

Synthetic peptide from the internal region of human NRAS protein, conjugated to KLH

Application

The antibody may be used in various immunochemical techniques including immunoblotting (predicted ∼21 kDa) and immunohistochemistry. Detection of the NRAS band by Immunoblotting is specifically inhibited by the immunizing protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable