SAB4200466

Anti-Desmoglein 2 (DSG2) antibody, Mouse monoclonal

clone AH12.2, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-ARVC10, Monoclonal Anti-ARVD10, Monoclonal Anti-CDHF5, Monoclonal Anti-CMD1BB, Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse, Monoclonal Anti-HDGC, Monoclonal Anti-desmoglein 2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AH12.2, monoclonal
• mol wt: antigen ~150 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells; western blot: 0.5-1.0 μg/mL using HeLa total cell extracts
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DSG2(1829)

Description

General description

Monoclonal Anti-Desmoglein 2 (DSG2) (mouse IgG1 isotype) is derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells. Desmogleins are a family of cadherin proteins. There are four distinct desmoglein genes (DSG1-DSG4), each differentially expressed depending upon the type of tissue and the state of differentiation. Desmoglein-2 (DSG2) is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis and heart.

Immunogen

derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells.

Application

Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Desmogleins play an important role in the formation of desmosomes, cell to-cell adhesions, often found in both simple and squamous epithelium. These adhesions are important for ensuring tissue integrity and regulation of paracellular movement of solutes and restricting access of luminal pathogens to underlying tissue compartments. Desmoglein-2 (DSG2) has been shown to regulate numerous cellular processes, including proliferation and apoptosis. Intracellular fragments of DSG2 promote apoptosis in colonic epithelial cells. Mutations in this gene is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable